Table 1. Characterization of Candidate Genes.
| Category | Gene | Locus | Meana Col-0 | Mean C24 | Mean Aa | Mean Col-0 X Aa | Mean C24 X Aa | Seed Phenotype At x Aac |
|---|---|---|---|---|---|---|---|---|
| Seed transcription factors | FIS2 | AT2G35670 | 115.4 | 222.5 | 2.2 | 13.3 | 11.1 | ns |
| PHERES1 | AT1G65330 | 303.6 | 90.9 | 0.0 | 551.1 | 1368.1 | 9.9e | |
| TTG2 | AT2G37260 | 771.1 | 705.0 | 84.5 | 758.5 | 614.3 | 15.28d | |
| IKU1 | AT2G35230 | 200.0 | 171.0 | 44.5 | 488.0 | 519.6 | 7.1e | |
| IKU2 | AT3G19700 | 16.1 | 8.0 | 0.0 | 5.2 | 6.7 | ns | |
| AGL2 | AT1G15800 | 10.3 | 9.8 | 8.9 | 13.8 | 19.1 | ns | |
| AGL25 | AT5G10140 | 12.2 | 56.9 | 211.3 | 51.9 | 35.4 | ns | |
| AGL12 | AT1G71692 | 102.3 | 62.4 | 2.2 | 26.7 | 54.8 | ns | |
| AGL27 | AT1G77080 | 330.6 | 3.4 | 522.5 | 115.3 | 118.9 | ns | |
| AGL29 | AT2G34440 | 24.6 | 8.9 | 44.5 | 43.5 | 53.4 | ns | |
| AGL31b | AT5G65050 | 546.1 | 107.5 | 84.5 | 223.3 | 257.8 | ns | |
| AGL42 | AT5G62165 | 22.0 | 4.0 | 88.9 | 1.8 | 7.0 | ns | |
| AGL48b | AT2G40210 | 48.1 | 43.2 | 0.0 | 104.1 | 167.8 | ns | |
| AGL62 | AT5G60440 | 147.6 | 83.0 | 2.2 | 332.2 | 617.0 | 10.9d | |
| AGL87 | AT1G22590 | 50.8 | 107.7 | 2.2 | 85.4 | 131.3 | ns | |
| AGL96b | AT5G06500 | 109.5 | 96.5 | 0.0 | 147.0 | 224.9 | – | |
| VRN2b | AT4G16845 | 366.7 | 139.9 | 244.6 | 182.1 | 169.7 | ns | |
| VIM5 | AT1G57800 | 95.8 | 90.6 | 11.1 | 707.2 | 947.4 | ns | |
| HDG9 | AT5G17320 | 189.2 | 229.5 | 6.7 | 816.1 | 1179.9 | – | |
| HDG10 | AT1G34650 | 67.2 | 10.2 | 17.8 | 222.1 | 241.6 | – | |
| Defense response | PCC1 | AT3G22231 | 1.4 | 0.3 | 0.0 | 0.0 | 0.4 | ns |
| NPR1 | AT1G64280 | 43.4 | 18.2 | 37.8 | 18.1 | 30.5 | 1.8d | |
| NBS-LRR | AT3G44630 | 152.6 | 28.0 | 6.7 | 59.0 | 31.8 | ns | |
| RPS1 | AT3G07040 | 30.6 | 0.0 | 13.3 | 4.7 | 2.7 | ns | |
| EDS1 | AT3G48090 | 29.2 | 12.3 | 100.0 | 19.1 | 14.6 | ns | |
| CDR2b | AT5G33340 | 32.7 | 6.4 | 4.4 | 84.4 | 109.0 | ns | |
| NDR1 | AT3G20600 | 10.9 | 8.0 | 26.7 | 4.7 | 3.5 | ns | |
| SID2 | AT1G74710 | 78.3 | 67.3 | 229.0 | 125.6 | 156.8 | ns | |
ns, not significant; –, did not test mutant.
a
The mean number of read counts (normalized to library size) from two biological replicates.
b
Benjamini-Hochberg adjusted P value from differential expression analysis of Col-0 hybrid versus C24
hybrid in DESeq.
c
The percentage of live seed at maturity when crossed to A. arenosa.
d
Significant difference in live seed in comparison to Ler-0 × Aa (4.9% live seed, n = 832) in a Student’s t test at P < 0.05.
e
Significant difference in live seed in comparison to Col-0 × Aa (0.8% live seed, n = 3893) in a Student’s t
test at P < 0.05.