Figure 3.

Effects of the rid1-1 Mutation on Vegetative Development.

(A) The wild type (WT) and rid1-1 were grown at 22°C for 2 weeks and then at 22 or 28°C for an additional 2 weeks. Bar = 1 cm.

(B) Morphological appearance of leaves from plants grown at 22°C for 4 weeks. The two cotyledons are indicated as Cot. Bar = 1 cm.

(C) to (H) Detailed observation of the second true leaves of the wild type ([C] to [E]) and rid1-1 ([F] to [H]) grown at 22°C.

(C) and (F) Differential interference contrast images of stomatal apparatuses. The red arrow indicates abnormal guard cells. Bar = 50 µm.

(D), (E), (G), and (H) Cross sections of the second true leaves of wild-type and rid1-1 plants grown at 22°C. Resin-embedded tissues were sectioned and stained with toluidine blue O. ep, epidermis; pm, palisade parenchyma; sm, spongy parenchyma. Vein structures are magnified in (E) and (H). Bars = 25 µm.