Figure 2.

Expression and Phenotypic Characterization of mtv1 and nev/agd5 Single and Double Mutants.

(A) Expression of NEV/AGD5 as revealed by the ProAGD5:AGD5-GUS transgene.

(B) Expression of MTV1 as revealed by the ProMTV1:MTV1-GUS transgene. Lowercase roman numerals indicate tissue: (i) primary seedling root, (ii) 11 DAG plant, (iii) expanded leaf of soil-grown plant, (iv) immature seed at heart embryo stage, (v) heart stage embryo, (vi) mature embryo, (vii) inflorescence with flowers of different developmental stages; arrowhead marks abscission zone.

(C) Phenotype of 4-week-old wild-type, nev/agd5, mtv1, and nev/agd5 mtv1 double mutant plants. A magnified image of the boxed nev/agd5 mtv1 plant is shown below.

(D) Root length of 5 DAG seedlings, as a box plot; whiskers indicate population range; n > 40.

(E) Pollen viability is not altered in the mtv1 nev/agd5 double mutant as indicated by Alexander stain.

(F) Flower size is not altered in the mtv1 nev/agd5 double mutant.

(G) Mature siliques. Arrowheads mark nonabscised floral organs in nev/agd5 single and mtv1 nev/agd5 double mutants.

Bars = 100 µm in (i) and (vi), 2 cm in (ii) and (iii), 25 µm in (iv), (v), and (E).