Genetic Interactions of clps1 with other clp Mutants.
(A) Dosage effect of clpc2 on clpc1. clpc1-1 plants were crossed with clpc2-2, and progenies with homozygous clpc1-1 and either homozygous or heterozygous clpc2-2 are shown. Plants were grown for 45 d under 10/14-h light/dark cycle at 120 µmol photons m−2 s−1. We note that the reciprocal dosage effect was not observed (data not shown). wt, the wild type.
(B) Effect of clps1 on clpc1-1. Homozygous single and double mutant plants were grown for 37 d under 10/14-h light/dark cycle at 250 µmol photons m−2 s−1. No visible differences were observed.
(C) Effect of clps1 on clpc2-2. Homozygous single and double mutant plants and the wild type were grown on soil for 23 d under 10/14-h light/dark cycle at 120 µmol photons m−2 s−1. No visible differences were observed.
(D) Effect of clps1 on clpc1 clpc2. clpc1-1 clpc2-2 homozygous plants were crossed with clps1. Growth and development are shown for the resulting homozygous double and triple mutants grown for 45 d under 10/14-h light/dark cycle at 120 µmol photons m−2 s−1. No visible differences were observed.
(E) Effect of clps1 on clpd. Homozygous single and double mutants as well as wild-type plants were grown for 23 d on soil under 16/8-h light/dark cycle at 120 µmol photons m−2 s−1. No visible differences were observed.
(F) Effect of clps1 on clpr2-1. Homozygous single and double mutants were grown for 25 d on soil under 16/8-h light/dark cycle at 120 µmol photons m−2 s−1. No visible differences were observed.