Figure 3.

PDLP5 Is Essential for SA-Induced PD Closure and Callose Deposition.

(A) to (C) DANS dye loading assays showing impairment of induced PD closure response in pdlp5-1 upon either 100 μM SA treatment ([A] and [B]) or Pma infection (C). Representative confocal images of abaxial leaf surfaces show the effects of SA treatment on the extents of dye diffusion (A). WT, the wild type. Bars = 200 μm.

(D) Lack of SA-induced PD callose accumulation in pdlp5-1. More than five and three individual plants were used for DANS assays and aniline blue staining, respectively. At least two biological repeats were performed for quantification. All images were taken from leaf number 4 and leaf number 5. Levels not connected by same letters are significantly different at the α = 0.05 level based on LSD test following one-way ANOVA. Bars indicate se.

[See online article for color version of this figure.]