Figure 6.

NPR1 Is Required for PDLP5-Mediated PD Closure and Callose Deposition.

(A) Lack of PD closure response in npr1 and PDLP5npr1 to Pma infection.

(B) Basal PD callose level in npr1 and PDLP5npr1 is normal. Abaxial surfaces of the fourth and fifth leaves of 3.5-week-old plants were imaged by a confocal microscopy following aniline blue staining. WT, the wild type. Bars = 20 μm.

(C) PD permeability in npr1, PDLP5npr1, and PDLP5 is insensitive to 100 μM SA treatment. More than five and three individual plants were used per treatment for DANS assays and aniline blue staining, respectively. At least two biological repeats were performed for quantification. Asterisks indicate a significant difference (P < 0.001) between two samples by t test.