Figure 3.

Gene Models for RLP1/ReMAX and Tomato Eix2.

(A) Three different gene models with start positions 1 at 2,269,893 or start 2 at 2,270,336 and different splicing options have been proposed for RLP1/At1g07390 (www.tair.org). The mutant lines rlp1-2 (SALK_116923) and rlp1-3 (SALK_049403C) have T-DNA insertions in predicted exons as indicated. Sequencing of Col-0 cDNAs resulted in a gene model with a start at position 2,269,764 (start 0) that we define as ReMAX. SP, signal peptide; LRR-NT, N terminus of the LRR domain; ID, island domain; JM, juxtamembrane domain; TM, transmembrane domain; CT, cytoplasmic tail.

(B) Gene constructs used for complementation experiments: Genomic and cDNA with the start positions of ReMAX (start 0) and RLP1.1/RLP1.3 (start 1) were cloned under the control of the 35S promoter. Where indicated, constructs were C-terminally fused in frame with a GFP tag. A similar construct with a GFP tag was produced for the RLP Eix2 (Ron and Avni, 2004) based on genomic DNA from tomato.