Figure 6.

A Doppia-Like Transposon in Bx10c Is Associated with Reduced HDMBOA-Glc Production and Increased Aphid Resistance in the NAM Parental Lines.

(A) Strategy for PCR analysis to amplify fragments that are specific for either the insertion or the deletion allele.

(B) Detection of the transposon knockout and functional alleles of Bx10c by PCR using primers TPA1, TPA2, and TPA3 (described in Supplemental Table 2 online).

(C) to (E) Comparison of DIMBOA-Glc content, HDMBOA-Glc content, and aphid reproduction in NAM parental lines, with and without the transposon insertion (mean ± se; n = 10 or 17). Asterisks indicate significant differences (*P < 0.05; two-tailed Student’s t test). FW, fresh weight.