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. 2013 Jul 25;8(7):e69939. doi: 10.1371/journal.pone.0069939

Figure 1. Glucose downregulates Rev-erb alpha gene expression in alphaTC1-9 cells and mouse pancreatic alpha cells.

Figure 1

(A) Gene expression of Rev-erb alpha in glucagon-secreting alphaTC1-9 cells, hypothalamus and liver (n = 5) The statistical significance was performed comparing the expression levels in alphaTC1-9 cells with the other tissues. (B) Intrinsic oscillations of Rev-erb alpha gene expression during 48 hours. AlphaTC1-9 cells were treated with 0.5 mM glucose (circles) and 11 mM glucose (square) for 48 hours (n = 6). The statistical significance was performed between the ZT (Zeitgeber Time) times comparing 0.5 mM and 11 mM. (C) Rev-erb alpha gene expression in alphaTC1-9 cells treated with 0.5 mM glucose and 11 mM glucose at ZT6 (n = 4–5). (D) Rev-erb alpha protein expression in alphaTC1-9 cells treated with 0.5 mM glucose and 11 mM glucose at ZT6 (n = 4). (E) Rev-erb alpha gene expression in primary mouse alpha-cells separated by FACS sorting and treated with 0.5 mM glucose and 11 mM glucose at ZT6 (n = 4). Alpha-cell enriched fraction: 80% alpha cells and 4% beta cells. Beta-cells enriched fraction: 75% beta-cells and no alpha-cells *p<0.05, **p<0.01, *** p<0.001. Data are expressed as mean ±S.E.M.