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. 2013 Jul 25;8(7):e69939. doi: 10.1371/journal.pone.0069939

Figure 4. Activation of Rev-erb alpha stimulates glucagon secretion and calcium signals in alphaTC1-9 cells and pancreatic alpha-cells.

Figure 4

(A) Glucagon secretion in alphaTC1-9 cells in the presence of 0.5 mM, 11 mM glucose and 10 µM of the Rev-erb alpha agonist GSK4112 (n = 7–8). (B) Intracellular calcium signals in alphaTC1-9 cells in the presence of 0.5 mM glucose and 10 µM of the Rev-erb alpha agonist GSK4112 (n = 42 cells). (C) Area under the curve calculated from experiments illustrated in 4B with alphaTC1-9 cells. (D) Intracellular calcium signals in alphaTC1-9 cells in the presence of 0.5 mM glucose and 10 µM of the Rev-erb alpha agonist GSK4112 (n = 24 cells). (E) Intracellular calcium measurements in primary mouse alpha cells in the presence of 0.5 mM glucose and 10 µM of Rev-erb alpha agonist GSK4112 (n = 14 cells). (F) Area under the curve calculated from experiments illustrated in 4E with primary alpha-cells. *p<0.05, **p<0.01, *** p<0.001. Data are expressed as mean ±S.E.M.