Figure 7. Activation of AMPK prevents glucose inhibition of Rev-erb alpha expression via Nampt-Sirt1 pathway in alphaTC1-9 cells.

(A) Nampt gene expression in alphaTC1-9 cells treated with 0.5 mM, 11 mM glucose or 500 µM Metformin for 6 hours (n = 5–6) and (B) 24 hours (n = 5–6). (C) Sirt-1 gene expression in alphaTC1-9 cells treated with 0.5 mM, 11 mM glucose or 500 µM Metformin for 6 hours (n = 5–6) and (D) 24 hours (n = 5–6). (E) PGC-1 alpha gene expression in alphaTC1-9 cells treated with 0.5 mM, 11 mM glucose or 500 µM Metformin for 6 hours (n = 5) and (F) 24 hours (n = 5–6). (G) AMPK phosphorylation (Thr¹⁷²) in alphaTC1-9 cells treated with 0.5 mM, 11 mM glucose or 500 µM Metformin for 6 hours. (H) NAMPT protein expression in alphaTC1-9 cells treated with 0.5 mM, 11 mM glucose or 500 µM Metformin for 6 hours. *p<0.05. Data are expressed as mean ±S.E.M.