Table 1. Quantitative Real-Time PCR primers.
NAME | Accession No. | Sense Primer (5′-3′) | Antisense primer (5′-3′) |
Rplp0 | NM_007475 | GAGGAATCAGATGAGGATATGGGA | AAGCAGGCTGACTTGGTTGC |
Vamp3 | NM_009498 | CTCACCAAGGCATCAGTCTG | ATTCTAAGAGCACCAGGCATC |
Munc18 | NM_001113569 | GGACTG AAG AGC GTC GTG TG | TTG GTG GTA AAC TCA TCT AAC AGC |
SNAP25 | NM_011428 | GTC TTT CCT TCC CTC CCT ACC | AGT CAG TGG TGC TTC GTT AAA |
Syntaxin 1a | NM_016801 | GATGAG AAGACAAAG GAGGAACTG | ATG AGC GGT TCA GAC CTT CC |
Nampt | NM_021524.2 | GAGACTGCCGGCATAGGGGC | GGTACTGTGCTCTGCCGCTGG |
Sirt1 | NM_001159589.1 | CCTGACTTCAGATCAAGAGA | TGTCTCCACGAACAGCTTCA |
PGC1alpha | NM_0089042 | AGCCGTGACCACTGACAACGAG | GCTGCATGGTTCTGAGTGCTAAG |
Rev-erb alpha | NM_145434 | GGTGCGCTTTGCATCGTT | GGTTGTGCGGCTCAGGAA |
Clock | NM_007715 | TTGCTCCACGGGAATCCTT | GGAGGGAAAGTGCTCTGTTGTAG |
Per2 | NM_011066 | ATGCTCGCCATCCACAAGA | GCGGAATCGAATGGGAGAAT |
Cry1 | NM_007771 | CTGGCGTGGAAGTCATCGT | CTGTCCGCCATTGAGTTCTATG |
Cry2 | NM_009963 | AGCCCAGGCCAAGAGGAA | GTTTTTCAGGCCCACTCTACCTT |
Bmal1 | NM_007489 | GGACTTCGCCTCTACCTGTTCA | AACCATGTGCGAGTGCAGGCGC |
Glucagon | NM_008100 | GGCTCCTTCTCTGACGAGATGAGCAC | CTGGCACGAGATGTTGTGAAGATGG |
Alas-1 | NM_020559 | ATCATCTCTGGGACGCTTGGTA | GGACGGTGTCGATCAGCAA |