Figure 2. Effect of BP on the expression of adipogenic genes in 3T3-L1 adipocytes.

3T3-L1 preadipocytes were differentiated into adipocytes in DMI medium in the absence or presence of 50 µg/mL or 200 µg/mL BPE for 4 or 7 days. (A) BPE inhibited the expression of adipocyte-specific transcription factors during differentiation. The gene expression analysis was performed by RT-PCR, and all of the gene transcripts were normalized using β-actin as a control. All of the experiments were performed in three independent experiments. Bars with different letters are significantly different (p<0.05) as determined by Duncan's multiple range test. (B) BP reduced the expression of adipogenesis-related genes in 3T3-L1 adipocytes. Total cell lysates were isolated from 3T3-L1 adipocytes at day 4 or day 7 after induction of differentiation. Western blotting analysis was performed as described in the Materials and Methods.