Table 2. Frequency of different morphological defects in embryos at E8.5 from matings of Tbx20 +/- ; Tbx3 +/- double heterozygotes*.
| Genotypes |
|||||||
|---|---|---|---|---|---|---|---|
| Stage | Morphology | wt** | Tbx20 -/- ; Tbx3 +/+ | Tbx20 -/- ; Tbx3 +/- | Tbx20+/+ ;Tbx3 -/- | Tbx20 +/- ; Tbx3 -/- | Tbx20 -/- ; Tbx3 -/- |
| E8.5 | Normal | 89 | 3*** | 2*** | 1*** | 0 | 1*** |
| Abnormal. looping | 0 | 14 | 14 | 0 | 0 | 0 | |
| Abnormal looping / delay | 0 | 3 | 3 | 0 | 0 | 3 | |
| Abnormal looping / dead | 0 | 2 | 0 | 0 | 0 | 0 | |
| Delay | 6 | 1 | 6 | 7 | 4## | 3 | |
| Dead# | 1 | 0 | 3 | 0 | 0 | 1 | |
| Total (n=167) | 96 | 23 | 28 | 8 | 4 | 8 | |
Double heterozygotes were either intercrossed or crossed to single heterozygotes.
wt, wild type; this group includes wild type and heterozygotes for one or both alleles.
Embryos were at the cardiac crescent stage, prior to heart looping.
Approximate time of death between E8.0 and E8.5; too degenerate to score for heart phenotype.
One of these embryos had a yolk sac defect.