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Concentration-dependent binding of recombinant P. falciparum 3D7 PFD1235w DBLβ3_D4 to ICAM-1-FcHEK239, ICAM-1-FcCOS-7 and ICAM-1-FcNS0 (R&D Systems) by ELISA. The binding of DBLβ3_D4 to ICAM-1-FcHEK239 was repeated in three independent experiments (mean and standard deviation shown) while the assay using ICAM-1-FcCOS-7 and ICAM-1-FcNS0 (R&D Systems) was done one time each.
