Table 2.
PCR and DNA primers sequencing of the three ESBL genetic markers
| Primer | Sequence | Size (bp) | Reference |
|---|---|---|---|
| Core TEM F |
CAGCGGTAAGATCCTTGAGA |
643 |
[9] |
| Core TEM R |
ACTCCCCGTCGTGTAGATAA |
||
| Core SHV F |
GGCCGCGTAGGCATGATAGA |
714 |
|
| Core SHV R |
CCCGGCGATTTGCTGATTTC |
||
| Core CTXM F |
AACCGTCACGCTGTTGTTAG |
766 |
|
| Core CTXM R |
TTGAGGCGTGGTGAAGTAAG |
||
| Upstream TEM F Upstream TEM R |
TGAAGACGAAAGGGCCTCCTG ACTCCCCGTCGTGTAGATAA |
109 |
Newly designed primers in GDDRP Molecular Laboratoryfor this study |
| Upstream SHV F Upstream SHV R |
CGTTWTDTTCGCCKGTGT CGAGTAGTCCACCAGATCCTG |
74 |
|
| Upstream XTXM-F Upstream CXTXM-R |
ATGGTTAAAAAATCACTG ACGTTATCGCTGTACTGTA |
55 |
|
| Downstream TEM F Downstream TEM R |
CAGCGGTAAGATCCTTGAGA TAATCAGTGAGGCACCTATCTC |
109 |
|
| Downstream SHV F Downstream SHV R |
GGCCGCGTAGGCATGATAGA CCCGGCGATTTGCTGATTTC |
74 |
|
| Downstream CTXM-F Downstream CTXM-R | TACAGTACAGCGATAACGTGG CCGTTTCCGCTATTACAAA | 55 |