Fig. 3.

Ifenprodil, a GluN2B selective antagonist of NMDARs, inhibits intracellular Ca²⁺ responses induced by NMDA. (A) Ca²⁺ responses measured upon application of 30 μM NMDA following by the addition 10 μM ifenprodil (the application episodes are marked with lines above the traces). Neurons were loaded with Fluo-3 (ordinate, relative fluorescence intensity). Each trace represents the response of one neuron. (B) Ca²⁺ responses measured upon application of 30 μM NMDA with 10 μM ifenprodil following by ifenprodil washout (the application episodes are marked with lines above the traces). Neurons were loaded with Fluo-3 (ordinate, relative fluorescence intensity). Each trace represents the response of one neuron. Four experiments were performed.