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. 2013 Jul 25;8(7):e68945. doi: 10.1371/journal.pone.0068945

Table 1. Diagnostic value comparison between methods cell line DNA analysis.

Labs (n) Samples (n) Analytical failures (n) Success rate in truenegative (%) Success rate in true positive (%)
Dilutions
All 100% 50% 25% 5%
Direct sequencing 15 1260 4 (3‰) 98.9 76 99 99 87.0 38
Taqman 8 672 11 (1.6%) 99.0 92.3 95.8 100 99.3 76.4
Snapshot 7 588 4 (7‰) 98.8 89.7 95.2 100 93.7 73.8
Pyrosequencing 5 420 6 (14‰) 95 96.6 100 100 100 89.7
HRM and sequencing 5 420 0 100 78.0 100 98.9 88.9 40.0
MASA 1 84 0 100 92.5 100 100 100 72.2
Scorpion 1 84 0 100 100 100 100 100 100
HRM+Taqman 1 84 0 100 100 100 100 100 33.3
PNA° based methods* 1+1 168 0 100 100 100 100 100 100

°PNA Peptide nucleic acid; *PNA was used with taqman probes or with allele specific PCR.