Table 1. Diagnostic value comparison between methods cell line DNA analysis.
Labs (n) | Samples (n) | Analytical failures (n) | Success rate in truenegative (%) | Success rate in true positive (%) | |||||
Dilutions | |||||||||
All | 100% | 50% | 25% | 5% | |||||
Direct sequencing | 15 | 1260 | 4 (3‰) | 98.9 | 76 | 99 | 99 | 87.0 | 38 |
Taqman | 8 | 672 | 11 (1.6%) | 99.0 | 92.3 | 95.8 | 100 | 99.3 | 76.4 |
Snapshot | 7 | 588 | 4 (7‰) | 98.8 | 89.7 | 95.2 | 100 | 93.7 | 73.8 |
Pyrosequencing | 5 | 420 | 6 (14‰) | 95 | 96.6 | 100 | 100 | 100 | 89.7 |
HRM and sequencing | 5 | 420 | 0 | 100 | 78.0 | 100 | 98.9 | 88.9 | 40.0 |
MASA | 1 | 84 | 0 | 100 | 92.5 | 100 | 100 | 100 | 72.2 |
Scorpion | 1 | 84 | 0 | 100 | 100 | 100 | 100 | 100 | 100 |
HRM+Taqman | 1 | 84 | 0 | 100 | 100 | 100 | 100 | 100 | 33.3 |
PNA° based methods* | 1+1 | 168 | 0 | 100 | 100 | 100 | 100 | 100 | 100 |
°PNA Peptide nucleic acid; *PNA was used with taqman probes or with allele specific PCR.