. 2013 Jul 25;8(7):e69080. doi: 10.1371/journal.pone.0069080

Table 4. Associations between candidate SNPs, season-standardized 25(OH)D levels, and the risks cardiovascular diseases.

						Risk of myocardial Infarction^†				Risk of stroke^†				Risk of CVD (composite)^†
SNP, locus*	Allele	N subcohort	Mean 25(OH)D	P**	R²	N cases	HR	95% CI	P	N cases	HR	95% CI	P	N cases	HR	95% CI	P
rs1155563,	T/T	1059	49.7			281	Ref.			243	Ref.			524	Ref.
GC	T/C	832	45.1			224	1.07	(0.86-1.33)		185	0.99	(0.79-1.24)		409	1.05	(0.88-1.25)
	C/C	164	42.4	<0.01	2.1	36	0.80	(0.53-1.21)	0.73	32	0.90	(0.59-1.37)	0.69	68	0.84	(0.60-1.18)	0.72
rs2282679,	A/A	1058	50.0			288	Ref.			249	Ref.			537	Ref.
GC	A/C	827	45.0			216	0.97	(0.77-1.21)		183	0.91	(0.73-1.14)		399	0.94	(0.79-1.13)
	C/C	166	40.3	<0.01	3.0	38	0.85	(0.57-1.27)	0.48	31	0.85	(0.56-1.31)	0.32	69	0.85	(0.61-1.19)	0.31
rs3829251,	G/G	1415	48.2			380	Ref.			314	Ref.			694	Ref.
DHCR7/	G/A	594	45.1			150	1.04	(0.83-1.31)		140	1.15	(0.91-1.45)		290	1.09	(0.90-1.31)
NADSYN1	A/A	60	44.0	<0.01	0.7	15	1.17	(0.63-2.17)	0.61	11	1.0	(0.50-1.98)	0.34	26	1.09	(0.65-1.83)	0.38
rs12785878,	T/T	1081	49.0			290	Ref.			241	Ref.			531	Ref.
DHCR7/	T/G	806	46.2			210	1.04	(0.84-1.29)		181	1.05	(0.84-1.31)		391	1.06	(0.89-1.27)
NADSYN1	G/G	172	40.3	<0.01	1.7	42	1.12	(0.76-1.65)	0.55	42	1.23	(0.83-1.81)	0.35	84	1.20	(0.87-1.65)	0.38
rs6599638,	G/G	555	46.3			135	Ref.			127	Ref.			262	Ref.
C10orff88	G/A	1020	47.6			268	1.10	(0.86-1.41)		231	1.02	(0.79-1.31)		499	1.07	(0.87-1.31)
	A/A	488	47.1	1.0	0.0	141	1.14	(0.85-1.52)	0.36	106	0.92	(0.68-1.23)	0.56	247	1.05	(0.83-1.33)	0.70
rs10741657,	G/G	768	46.2			208	Ref.			183	Ref.			391	Ref.
CYP2R1	G/A	966	47.0			251	1.07	(0.86-1.34)		212	1.03	(0.82-1.30)		463	1.06	(0.88-1.28)
	A/A	317	49.2	0.10	0.3	87	0.94	(0.69-1.28)	0.87	66	0.81	(0.60-1.24)	0.33	153	0.90	(0.69-1.16)	0.62
rs10877012,	G/G	919	48.1			243	Ref.			213	Ref.			456	Ref.
CYP27B1	T/G	910	46.7			251	1.10	(0.89-1.37)		203	1.00	(0.80-1.25)		454	1.11	(0.89-1.40)
	T/T	238	45.3	0.16	0.3	49	0.79	(0.55-1.13)	0.59	47	0.86	(0.59-1.23)	0.53	96	1.07	(0.86-1.35)	0.38
rs6013897,	T/T	1315	47.7			337	Ref.			276	Ref.			613	Ref.
CYP24A1	T/A	680	46.3			185	1.07	(0.86-1.34)		164	1.16	(0.92-1.45)		349	1.09	(0.90-1.31)
	A/A	67	46.5	0.99	0.1	27	1.50	(0.90-2.52)	0.18	22	1.54	(0.90-2.63)	0.07	49	1.48	(0.96-2.30)	0.07
SNP Score^‡	Risk alleles
	0	546	52.4			159	Ref.			127	Ref.			286	Ref.
	1	865	47.3			210	0.88	(0.69-1.13)		190	1.00	(0.77-1.29)		400	0.94	(0.76-1.13)
	2	462	43.9			123	0.93	(0.70-1.25)		113	1.05	(0.78-1.42)		236	1.01	(0.79-1.29)
	≥3	161	38.9	<0.01	4.4	41	1.07	(0.71-1.62)	1.0	30	0.90	(0.57-1.42)	0.95	71	1.00	(0.71-1.42)	0.90

Percentages of missing values for single SNPs: rs1155563: 3.5%, rs2282679:3.4%, rs3829251:2.8%, rs6013897: 2.9%, rs6599638:3.0%, rs10741657:3.4%, rs10877012:3.0%, and rs12785878:3.3%;

^**

P for linear trend calculated by ANCOVA within Generalized Linear Models modeling the number of risk alleles as continuous score, adjusted for age, sex and study center; Initial p values were multiplied by 8 to account for the number of tested SNPs;

^†

Calculated by Cox regression analyses stratified by age and center, and adjusted for sex; P for linear trend calculated modeling the number of risk alleles as continuous score; P values from Cox regressions were not corrected for multiple testing because all associations were non-significant;

^‡

Consisting of rs2282679 and rs12785878; rs1155563 and rs2282679 in the GC locus, as well as rs3829251 and rs12785878 in the DHCR7/NADSYN1 loci were in strong linkage disequilibrium (R²>0.8);

No deviation from Hardy-Weinberg-Equilibrium was detected for any of the SNPs