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. 2013 Jul 25;8(7):e69902. doi: 10.1371/journal.pone.0069902

Figure 2. CrCDPK3 is present in the flagella of C. reinhardtii.

Figure 2

(A) Schematic diagram of CrCDPK3 gene showing exons (grey) and introns (white). (B) Immunoblot analysis of Chlamydomonas cell lysates, bacterial expressed GST-CrCDPK3 and GST shows that anti-CrCDPK3 antibody is specific. Molecular weights are given in kilo-daltons. (C) CrCDPK3 is present in the cell body and flagella evidenced by immuoblotting with anti-CrCDPK3 antibody. 1 x indicates that approximately two flagella were loaded per cell body. 50 x indicates equal flagellar and cell body protein. (D) Isolated flagella (F), membrane/matrix (M and M) and axonemal (Ax) fractions were analyzed by immunoblotting with antibodies as indicated. (E) Immunostaining of cells expressing CrCDPK3-HA (a) or not (b) with antibodies against 3xHA tag and α-tubulin. Bars, 5 µm.