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. 2013 Jul 25;8(7):e69902. doi: 10.1371/journal.pone.0069902

Figure 6. CrCDPK3 is defective in flagellar elongation induced by LiCl.

Figure 6

(A) Flagellar length increase after treatment with 25 mM LiCl for wild type and RNAi strains. (B) Differential interference contrast images of cells before treatment (a) and 180 min after treatment (b–f). b, elongated normal flagella; c, flagellum with one small bulb proximal to flagellar tip (arrow); d, one flagellum curled at the flagellar base; e, two curled flagella with one remaining flagellar tip (arrow); f, loss of curled flagella. (C) Statistical presentation of cells with different forms of flagella after LiCl treatment for wild type cells, and (D) for RNAi strain C60, and (E) for RNAi strain C70. (F) Increase of IFT proteins during flagellar elongation induced by LiCl. Flagella isolated from wild type or RNAi strains after 30 min treatment were analyzed by immunoblotting with antibodies against IFT139, IFT motor protein Fla10, and α-tubulin, which was used as loading control.