Table 1. Sequences of phage clones isolated after 3-round of panning and IC50 values of the peptides analyzed.
Clone ID | Sequence | # | AlphaScreen | Enzyme inibition |
B | LPIFKVDFGDHSPFT | nd | nd | nd |
C | ARMFLLFLMRCIGCY | 565* | >4.83 µM | a |
D, H | SHSFSVGSG DHSPFT | 562 | nd | 661 µM (76 µM)† |
SHSFSVGSG DHSPFT | (562)4 | nd | 311 µM | |
E, F | SHSFSVGSGSGDHSP | nd | nd | nd |
G | LSFFSCWLRRSFSLT | 564* | 1.72 µM | b |
I | EVPRLSLLAVFLVVM | nd | nd | nd |
J | EVPRLSLLAVFLCNG | nd | nd | nd |
K, L | EVPRLSLLAVFLVAN | nd | nd | nd |
M | GRFLTGGTGRLLRIS | 563 | 708 nM | c |
Cys(Acm) in peptides 564 and 565.
Ki value from the Cheng-Prussof equation.
, no inhibition observed at 14.5 µM.
, no inhibition observed at 55.5 µM.
, no inhibition observed at 1 mM.
nd, not determined.