Figure 7. Effect of estradiol on nucleotidase gene expression in resting blood-derived CD4+T cells.
(A) Relative gene expression of nucleotidases in blood-derived CD4+T Cells derived from 6 donors. Data is shown as the ratio of the nucleotidase (NT) gene expression to the expression of β-actin. (B–D) Effect of estradiol treatment on fold change in mRNA expression in CD4+T cells treated with 5×10−8 M estradiol for (B) 2, (C) 6 and (D) 24 h. Control (no estradiol) is assigned a value of 1 (dashed line). UD = undetectable. The mean and SEM from 6 donors are shown.