. 2012 Oct 30;3(4):255–265. doi: 10.1007/s13205-012-0099-8

Table 4.

Production of lipase by different microbial species grown in fish processing by-products

Fish raw materials	Preparation of the growth media	Microbial strains	Lipase activity (U/ml)	References
Defatted tuna by-products	Extraction with chloroform/methanol^a	Staphylococcus epidermidis CMST Pi 2	12.63	Esakkiraj et al. (2010a)
Defatted tuna by-products	Extraction with chloroform/methanol^a	Staphylococcus epidermidis CMST Pi 2	14.20	Esakkiraj et al. (2010a)
Tuna by-products	Raw materials were cooked, bones were removed, pressed to remove water and fat, pressed, minced and dried (80 °C, 24–48 h)^a	Staphylococcus epidermidis CMST Pi 2	8.17	Esakkiraj et al. (2010a)
Acid-hydrolyzed tuna waste	Method described by Gao et al. (2006)^a	Staphylococcus epidermidis CMST Pi 2	8.03	Esakkiraj et al. (2010a)
Alkali-hydrolyzed tuna waste	Method described by Batista (1999)^a	Staphylococcus epidermidis CMST Pi 2	8.13	Esakkiraj et al. (2010a)
Shrimp by-products	Raw materials were boiled (100 °C for 20 min) in water and supernatants were recuperated by centrifugation^b	Staphylococcus xylosus	19–28	Ben Rebah et al. (2008)
Cuttlefish by-products	Raw materials were boiled (100 °C for 20 min) in water and supernatants were recuperated by centrifugation^b	Staphylococcus xylosus	5–9.50	Ben Rebah et al. (2008)
Tuna by-products	Raw materials were boiled (100 °C for 20 min) in water and supernatants were recuperated by centrifugation^b	Staphylococcus xylosus	0–4	Ben Rebah et al. (2008)
Sardine by-products	Raw materials were boiled (100 °C for 20 min) in water and supernatants were recuperated by centrifugation^b	Staphylococcus xylosus	0–3	Ben Rebah et al. (2008)
Cod liver oil	1 % of fish oil added to the basal medium	Staphylococcus epidermidis CMST Pi 1	14.8	Esakkiraj et al. (2010b)

^aThe obtained fish powder was added to the basal medium at different proportions

^bThe supernatant was used as a nutrient source for lipase production