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Binding inhibition curves for 125I-HEAT (200 pM) binding to WT (0.2 µg, black), F1875.41A (0.15 µg, light blue), the double S1885.42,S1925.46-AA (0.3 µg, dark blue), F1935.47A (0.25 µg, green), F2816.44A (0.15 µg, orange), F2886.51A (0.2 µg, red), M2926.55A (0.2 µg, purple), F3087.35A (0.1 µg, brown), F3127.39A (0.8 µg, grey), n = 3–4.
