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. 2013 Jul 25;8(7):e69468. doi: 10.1371/journal.pone.0069468

Figure 5. Effects of KMUP-1 on mRNA expression of osteoclastogenesis-related genes.

Figure 5

Total RNA was extracted from RAW264.7 cells cultured for 24 h in the presence of RANKL (10 ng/ml) and KMUP-1 (0–10 µM), or with pretreatment of PKA inhibitor H89 (10 µM) and PKG inhibitor KT5823 (3 µM) for 30 min. The mRNA expression of the indicated genes was analyzed by real time RT-PCR. Each value represents the mean ± S.E.M. of three independent experiments, with triplicate determinations in each experiment. ## P<0.01 compared with control; *P<0.05 compared with RANKL alone; + P<0.05 compared with KMUP-1 10 µM plus RANKL.