Skip to main content
. 2013 Jul 25;8(7):e69468. doi: 10.1371/journal.pone.0069468

Figure 9. Effects of KMUP-1 on RANKL-induced activations of MAPKs and Akt pathways.

Figure 9

(A) Time course analysis of RANKL-induced phosphorylation of MAPKs and Akt showed that their activations were maximized at 15 min or 30 min, respectively. (B–E) RAW264.7 cells were pretreated with KMUP-1 for 24 h followed by stimulation with RANKL (10 ng/ml) for 15 min (MAPKs) or 30 min (Akt). The cell lysates were analyzed by Western blotting. Each value represents the mean ± S.E.M. of three independent experiments, with triplicate determinations in each experiment. ## P<0.01 compared with control; *P<0.05, **P<0.01 compared with RANKL alone.