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. 2013 Apr 25;90(8):1101–1109. doi: 10.1007/s11746-013-2252-x
Time (min) Acetonitrile Water Isopropanol Flow rate (mL/min)
0 20 80 0 0.30
15 80 5 15 0.30
30 82 0 18 0.30
50 100 0 0 0.60
56 100 0 0 0.60
57 20 80 0 0.30
60 20 80 0 0.30