Table 1.
Parental-type molecular variant mean values for each marker, most significant marker or marker interval, LOD, measure of additive effects and percent regression effect explained from Multiple QTL Mapping using MapQTL in the Carberry/AC Cadillac doubled haploid population for DArT® and SSR markers with stem rust severity and infection response from the field in Njoro, Kenya and Swift Current, Canada, and for seedling stem rust infection type against race TTKSK from containment growth chamber trials in Morden, Canada
| Chromosome | QTL | Trait | Environment | Markera/Marker interval | LODb score | Mean AC Cadillac molecular variant | Mean Carberry molecular variant | Additivec effect | PVd % |
|---|---|---|---|---|---|---|---|---|---|
| 2B | QSr.spa-2B.1 | Stem rust severity | Kenya 2010 | wPt-6832 | 7.4 | 6.4 | 14 | −3.8 | 10.3 |
| QSr.spa-2B.1 | Stem rust severity | Kenya 2011 | tPt-9065 | 7 | 10.3 | 12.6 | −1.1 | 9.7 | |
| QSr.spa-2B.1 | Stem rust infection response | Kenya 2011 | wPt-6832 | 3.7 | 1.7 | 3.4 | −0.8 | 6.3 | |
| QPbc.spa-2B | PBC | Kenya 2010 | Xwmc770 | 3.2 | 1.7 | 1.5 | 0.1 | 4.9 | |
| QPbc.spa-2B | PBC | Kenya 2011 | Xwmc770 | 7.1 | 1.3 | 1.1 | 0.1 | 11.7 | |
| 3B | QSr.spa-3B.1 | Stem rust infection response | Canada 2011 | Xbarc147 | 3 | 1.9 | 2.4 | −0.2 | 4.6 |
| QPbc.spa-3B | PBC | Kenya 2010 | X3B042G11 | 8.1 | 1.8 | 1.4 | 0.2 | 13.7 | |
| QPbc.spa-3B | PBC | Canada 2012 | wPt-744251 | 12 | 1.3 | 1 | 0.2 | 18 | |
| 4B | QSr.spa-4B.1 | Stem rust infection type | Morden 2012 | wPt-744434–Xwmc617 | 3.1 | 5.9 | 4.2 | 0.8 | 2.8 |
| QSr.spa-4B.1 | Stem rust infection response | Kenya 2009 | wPt-744434–Xwmc617 | 2.9 | 4.8 | 3.7 | 0.5 | 2.4 | |
| QSr.spa-4B.1 | Stem rust severity | Kenya 2009 | wPt-744434 | 4 | 12.3 | 7.9 | 2.2 | 5.8 | |
| QSr.spa-4B.1 | Stem rust severity | Kenya 2010 | wPt-744434 | 4.5 | 13.4 | 7 | 3.2 | 6.1 | |
| QSr.spa-4B.1 | Stem rust severity | Canada 2011 | wPt-744434–Xwmc617 | 3.4 | 14.3 | 9 | 2.6 | 5.3 | |
| QSr.spa-4B.1 | Stem rust infection response | Canada 2011 | wPt-744434–Xwmc617 | 3 | 2.6 | 2 | 0.3 | 4.7 | |
| QSr.spa-4B.1 | Stem rust infection type | Morden combined | wPt-733745 | 3 | 6.3 | 4.8 | 0.8 | 5.1 | |
| 5A | QSr.spa-5A | Stem rust infection response | Canada 2011 | wPt-5408 | 2.9 | 2.5 | 2 | 0.2 | 4.5 |
| QSr.spa-5A | Stem rust severity | Canada 2012 | wPt-2175 | 3.2 | 3.7 | 2.1 | 0.8 | 5.5 | |
| QSr.spa-5A | Stem rust severity | Canada Combined | wPt-666793 | 3.1 | 8.2 | 6 | 1.1 | 5.3 | |
| QSr.spa-5A | Stem rust infection response | Canada combined | wPt-5256 | 3.4 | 2 | 1.6 | 0.2 | 5.9 | |
| 5B | QSr.spa-5B.1 | Stem rust severity | Kenya 2009 | wPt-9205 | 3.6 | 8.2 | 12 | −1.9 | 4.9 |
| QSr.spa-5B.1 | Stem rust infection response | Canada 2011 | wPt-5792 | 3.6 | 2 | 2.5 | −0.3 | 5.4 | |
| 6A | QPbc.spa-6A | PBC | Canada 2012 | wPt-2014–tPt-8557 | 3.2 | 1.1 | 1.3 | −0.1 | 4.4 |
| 6D | QSr.spa-6D | Stem rust infection type | Morden 2012 | wPt-741955 | 34.7 | 2.5 | 7.5 | −2.5 | 42.8 |
| QSr.spa-6D | Stem rust infection type | Morden 2009 | wPt-1695 | 39.2 | 2.7 | 7.5 | −2.4 | 48.8 | |
| QSr.spa-6D | Stem rust infection response | Kenya 2009 | wPt-1695 | 39.8 | 2.3 | 6.1 | −1.9 | 47.8 | |
| QSr.spa-6D | Stem rust severity | Kenya 2009 | wPt-664770 | 8.5 | 7 | 13.2 | −3.1 | 12.8 | |
| QSr.spa-6D | Stem rust severity | Kenya 2010 | wPt-741955 | 2.9 | 7.8 | 12.6 | −2.4 | 3.9 | |
| QSr.spa-6D | Stem rust infection response | Kenya 2010 | wPt-741955 | 13.4 | 1.6 | 3.4 | −0.9 | 21.1 | |
| QSr.spa-6D | Stem rust infection response | Canada 2012 | wPt-1695 | 3.4 | 1.1 | 1.4 | −0.2 | 5.8 | |
| QSr.spa-6D | Stem rust infection type | Morden combined | wPt-741955 | 9.4 | 3.8 | 6.4 | −1.3 | 15 | |
| QSr.spa-6D | Stem rust infection response | Kenya combined | wPt-741955 | 6.7 | 2.6 | 3.7 | −0.5 | 11.1 | |
| 7B | QSr.spa-7B | Stem rust infection type | Morden 2012 | wPt-3939 | 8.6 | 3.8 | 5.8 | −1 | 7.1 |
| QSr.spa-7B | Stem rust infection type | Morden 2009 | wPt-3939 | 3.6 | 3.4 | 6.8 | −1.7 | 3.2 | |
| QSr.spa-7B | Stem rust severity | Kenya 2009 | wPt-3939 | 3.2 | 8.1 | 11.6 | −1.8 | 4.3 | |
| 7D | QSr.spa-7D | Stem rust infection type | Morden 2012 | Xwmc273 | 4.4 | 4.4 | 6 | −0.8 | 3.8 |
aMarker interval described by the markers which immediately flank the peak QTL response, or in the case of a single marker, the marker which is at the peak QTL response
bThe threshold to declare LOD scores significantly ranged from 2.9 to 3.0. All LOD scores reported are significant
cA positive additive effect indicates Carberry contributed to stem rust resistance and a negative additive effect indicates AC Cadillac contributed to stem rust resistance
dPV is the proportion of the phenotypic variance explained by the QTL