Figure 14.

Neither AtYSL4 nor AtYSL6 is able to complement fet3fet4. These experiments were performed as described (Chu et al. 2010). fet3fet4 yeast (derived from DEY1453) transformed with pGEV and pYES6/CT empty vector (top row) or constructs containing AtYSL3 (middle row), AtYSL4 (third row), and AtYSL6 (bottom row) were plated onto SD-TRP medium containing 50 mM Fe-Citrate (first column), 0 mM iron + 10 nM beta-estradiol (BE) (second column), 5 mM FeSO4 + 10 nM BE (third column), 5 mM Fe-NA complex + 10 nM BE (fourth column), or 5 mM Fe-NA complex without BE (last column). Pairs of spots correspond to 100 and 1000 fold dilutions of the original cultures. Plates were photographed after 10 days of growth.