Figure 9.

Response of Col-0, ysl4-2, ysl6-5 and ysl4ysl6 to Fe-citrate excess. (A) Plants were grown vertically for two weeks on medium containing either 500 mM Na-Citrate or 500 mM FeCitrate prior to photographing. (B) Plants were grown for 2 weeks on regular MS medium (no additives, pH 5.7) before measuring chlorophyll content. (C) Plants were grown for 2 weeks on MS medium containing 500 mM NaCitrate (pH 5.7) before measuring chlorophyll content. (D) Plants were grown for 2 weeks on MS medium that was adjusted to pH 5.7 after addition of 500 mM Fe-Citrate. Chlorophyll content was measured as in (B,C). For (B–D), chlorophyll content of three batches of seedlings was measured and averaged. Error bars indicate ±SD.