Figure 4.

Folate prevented homocysteine-mediated increases in p21/p27 cytosolic and RhoA localization by inactivating AKT1. Cells were treated as described for Figure 3A, but with an increased duration (6 or 9 h) of homocysteine challenge (A), or were transfected with AKT constructs as described in Figure 3B. The resulting cell lysates were fractionated and analysed for the cytosolic-nuclear distribution of p21 and p27 and cytosolic-membrane RhoA by Western blotting. Data were derived from three independent experiments and are presented as the mean ± SD *P < 0.05 vs. the control; ^#P < 0.05 vs. the homocysteine-challenged or CAAKT1 group.