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. 2013 Jul 12;169(7):1461–1476. doi: 10.1111/bph.12197

Figure 1.

Figure 1

EH-201 is a potent inducer of EPO expression. (A) The chemical structure of EH-201. (B, C) The EH–201-treated kidney slices and hepatocytes were analysed for EPO expression by Q-PCR and Western blotting. (D, E) Primary mice cardiomyocytes and (F, G) C2C12 myotubes were treated with EH-201, and the effects on EPO and EPOR expression were analysed by Q-PCR and Western blotting. (H) The bone marrow cells were incubated with EH-201 for 48 h, and the expression of EPO was detected by Q-PCR. (I) The bone marrow cells were incubated with EH-201, and the colonies were counted on day 9 for burst-forming units-erythroid (BFU-E). (J) The quantification of the differentiated erythroid progenitors was performed using a Hb colorimetric assay. The control represents vehicle treatment. The values are presented as the means ± SEM (n = 6 for each). *P < 0.01, *P < 0.05 versus control, Student's t-test.