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Potentiating effect of SKA-31 on relaxing responses in saphenous arteries of male (A–C) and female (D–F) mice of 12 (A, D), 34 (B, E) and 64 (C, F) weeks of age. Arteries were treated with either INDO (10 μM) and L-NAME (100 μM), or the combination of L-NAME (100 μM), INDO (10 μM) and SKA-31 (1 μM). Relaxing responses to ACh were recorded (0.01–10 μM) during PHE-induced (10 μM) contraction. Values are shown as means ± SEM (n = 4–5).
