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. 2013 Jul 12;169(7):1561–1573. doi: 10.1111/bph.12218

Figure 1.

Figure 1

The effect of short-time AG treatment on primary rat BECs. Increasing concentrations of PG and HG were used to study the cytotoxic effects on BECs. (A) WST-1 assay 45 min and 24 h after a 5 min AG treatment. Untreated cells served as negative control, cells treated with 1% Tx-100 for 6 h as positive control. For comparison, cells were treated with 0.7 and 1.4 M mannitol (M). All values presented are the means ± SEM, n = 3 for all groups. One-way anova followed by Dunnett's multiple comparison test revealed statistically significant decrease (a) between treatment groups and NaCl-treated group. (B) Apoptosis elisa 60 min after a 5 min AG treatment. Untreated cells served as negative control, DNA-histone complex served as positive control. All values presented are the means ± SEM, n = 6 for all groups. *P<0.05, significantly different from NaCl-treated group; one-way anova followed by Dunnett's multiple comparison test.