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. 2013 Jul 12;169(7):1635–1645. doi: 10.1111/bph.12219

Figure 1.

Figure 1

Inhibition of proliferation and cell cycle arrest is induced by TX 527 and bortezomib. SVEC-vGPCR cells were plated and incubated in serum-free DMEM for 24 h. After overnight growth, cells were treated with 10 nM TX 527 or vehicle (ctrl), bortezomib (0.5 nM) or combination of both TX 527 and bortezomib in DMEM 2% BSA for 24–48 h. (A) Proliferation assay was carried out using MTS as described in Methods section. Absorbance was measured at 490 nm. The data from three independent experiments are means ± SEM performed in triplicate. Significant differences between control and treated conditions are indicated. *P < 0.05, **P < 0.01. (B) Cells were then stained with propidium iodide and the distribution of cells in the different phases of the cell cycle was analysed by flow cytometry. The program CellQuest was used for acquisition and analysis of the FACS scans (C). A representative cell cycle analysis and percentages of each phase are shown. Data represent means ± SD of three independent experiments done in triplicate. Significant differences between cell cycle distribution of control and stimulated cells are indicated. *P < 0.05, **P < 0.01. G0/G1= cells in G0/G1 cell cycle phases; S = cells in S cell cycle phase; G2/M = cells in G2/M cell cycle phases.