Figure 2.

TX 527, similar to 1α,25(OH)₂D₃, inhibits NF-κB activity in SVEC-vGPCR. SVEC-vGPCR cells were treated with 10 nM 1α,25(OH)₂D₃ and TX 527 or vehicle (0.01% ethanol), bortezomib (0.5 nM), and combination of both TX 527 and bortezomib in DMEM 2% BSA for 48 h. TNFα-activated HeLa cell nuclear extracts and buffer for antibodies used alone were included in the assay as positive and negative controls respectively. NF-κB activity was measured by chemiluminescence reaction captured with a microplate reader as described in Methods section. The data from each experiment are means ± SD performed in duplicate. Significant differences between control (vehicle) and treated conditions are indicated. *P < 0.05, **P < 0.01.