Figure 4.
Effect of Gi2α ribozyme on ERK1/2 activation. Gi2α ribozyme (Rz) or non-cleaving Gi2α ribozyme (Rzm) complexed with DOTAP giving final ribozyme concentrations of 2.5 μM, or DOTAP without ribozyme, were added to hepatocyte cultures at 4–5 hours after the time of seeding. After 30 h of treatment the cell cultures were stimulated with PGF2α (10 μM) or EGF (10 nM) for 5 min before they were harvested and ERK1/2 activity assessed. Results are expressed as percent of untreated control and represent mean ± S.E.M. from three experiments.