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. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: Gastroenterology. 2011 Jul 18;141(4):1323–1333. doi: 10.1053/j.gastro.2011.07.005

Figure 2.

Figure 2

Figure 2

TNF-α effect on miR-122a expression, and effect of miR-122a over-expression on occludin 3′UTR. (a) Time course of TNF-α (10 ng/ml) effect on miR-122a expression as analyzed by real-time PCR. (b) MiR-122a binding site on occludin 3′UTR in various mammalian species. (c) Occludin 3′UTR region was cloned into pMIR-REPORT vector in 5′ to 3′ direction, and the effect of pre-miR-122a transfection on occludin 3′UTR was determined by luciferase assay. (d) MiR-122a binding site was deleted by site-directed mutagenesis, and effect of pre-miR-122a transfection on deletion construct was determined. * p < 0.01 vs control; ** p < 0.001 vs WT transfected with pre-miR-122a.