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. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: J Immunol. 2007 Apr 1;178(7):4641–4649. doi: 10.4049/jimmunol.178.7.4641

FIGURE 7.

FIGURE 7

A, Effect of IL-1β on occludin mRNA levels was assessed by real-time PCR. Filter-grown Caco-2 cells were treated with 10 ng/ml IL-1β for 16 h. B, Pretreatment with PDTC (100 μM) prevented the IL-1β-induced decrease in occludin mRNA levels. Data are represented as means of four replicates ± SE; *, p < 0.001 vs control. C, Effect of NF-κB inhibitor, PDTC (100 μM), and (D) NF-κB p65 depletion by siRNA on IL-1β-induced down-regulation of occludin protein expression. Caco-2 monolayers were treated with IL-1β for the 48-h experimental period. PDTC (100 μM) and NF-κB p65 siRNA significantly prevented the IL-1β-induced down-regulation in occludin protein expression.