Figure 2.

H&E images of positive controls for skin and adipose constructs after 2 and 8 days in culture. Skin constructs demonstrated a well-organized, multilayer epithelium by day 2 (a) that demonstrated all 4 morphological layers and a collagen lattice in which resident fibroblasts showed their characteristic spindled morphology. This epithelium was further developed by day 8 (c), where it showed a columnar basal cell layer and more complete development of the spinous cell layer. By day 8, cells populated the entire silk sponge in the adipose construct. Images are representative of n=2 per group.