Table 2.
Glycerol levels were greater in tri-layer groups but did not vary with media type. Glycerol levels were consistently greater in the tri-layer equivalent groups suggesting metabolic demands were not negatively impacted when all three layers were in contact.
| Glycerol in media | Tissue | |||
|---|---|---|---|---|
| Day | Media | Epidermal-dermal | Adipose | Tri-layer equivalent |
| 0 | Skin | ++ | + | ++ |
| 1:1 | + | + | ++ | |
| Adipose | + | ++ | ++ | |
| 2 | Skin | ++ | + | + |
| 1:1 | + | + | ++ | |
| Adipose | + | ++ | ++ | |
| 6 | Skin | + | + | ++ |
| 1:1 | + | + | ++ | |
| Adipose | + | + | ++ | |
| 8 | Skin | + | + | ++ |
| 1:1 | + | + | ++ | |
| Adipose | ++ | ++ | ++ | |
| Controls | Media only | Skin | − | − |
| 1:1 | − | − | ||
| Adipose | − | − | ||
(–) means no glycerol detected, (+/−) means borderline detection, (+) means low glycerol level, (++) means moderate glycerol level, and (+++) means high glycerol level. Levels are representative of n=2 per group.