Table 3.
Gene expression of various dermal and adipose markers. Dermal markers-collagen I and collagen IV increased from day 5 to 9. Fat markers remained consistent from day 5 to 9.
| Gene | Region | Primers (forward/reverse) | Time | Expression |
|---|---|---|---|---|
| Collagen I, a1 | Dermal | 5′ TCC CCA GCT GTC TTA TGG CT | Day5 | + |
| 5′ CAG GCA CGG AAA TTC CTC C | Day9 | ++ | ||
| Collagen IV, a1 | Dermal | 5′ CGC TTA CAG CTT TTG GCT CG | Day5 | ++ |
| 5′ GAC GGC GTA GGC TTC TTG AA | Day9 | +++ | ||
| Elastin | Dermal | 5′ GGT ATC CCA TCA AGG CCC C | Day5 | +/− |
| 5′ TTT CCC TGT GGT GTA GGG CA | Day9 | +/− | ||
| Fatty Acid Synthase (FAS) | Adipose | 5′ TGT GGA CAT GGT CAC GGA C | Day5 | + |
| 5′ GGC ATC AAA CCT AGA CAG GTC | Day9 | + | ||
| Fatty Acid Binding Protein (FABP4) | Adipose | 5′ AGC ACC ATA ACC TTA GAT GGG | Day5 | +/− |
| 5′ CGT GGA AGT GAC GCC TTT CA | Day9 | +/− | ||
| Glucose transporter 4 (Glut4) | Adipose | 5′ CTC AGC AGC GAG TGA CTG G | Day5 | ++ |
| 5′ CCC CAA TGT TGT ACC CAA ACT | Day9 | ++ |
(–) means no cDNA detected, (+/−) means borderline detection, (+) means low cDNA level, (++) means moderate cDNA level, and (+++) means high cDNA level. Levels are representative of n=2 per group.