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. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: Biochemistry. 2012 Jan 24;51(5):974–985. doi: 10.1021/bi201135s

Figure 5.

Figure 5

Comparison of peroxidase catalytic activity using So cyt c5 (4 μM) as the electron donor for (A) semi-reduced tag-free So CcP and (B & C) oxidized tag-free So CcP. Experiments are conducted in 5 mM MES, 5 mM HEPES, 10 mM NaCl, 1 mM CaCl2 (pH 6.0) at 23°C. In A & B, points represent the data and a line represents the linear rate used for Michaelis-Menten analysis. Pane C compares the experimental data (points) to the least-square fit of the data to the kinetic model (line) as described in the text. The inset in (C) shows the residuals of the least-square fit.