. Author manuscript; available in PMC: 2013 Jul 26.

Published in final edited form as: Biochemistry. 2012 Jan 24;51(5):974–985. doi: 10.1021/bi201135s

Table 5.

Kinetic parameters of both the oxidized and semi-reduced states of tag-free So CcP variants using So c5 as the electron donor.

			K_m for H₂O₂ (μM)	k_cat^obs (s⁻¹)	k_act (s⁻¹)	k₁ (s⁻¹)^*
Loop 1	P75T/H81K/E84Q	Oxidized	1.0 ± 0.3	0.3 ± 0.1	0.6 ± 0.2	0.56± 0.04
Loop 1	P75T/H81K/E84Q	Semi-reduced	0.4 ± 0.3	13 ± 1	n.a.	n.a.
Loop 3	M219Q/F247N	Oxidized	1.6 ± 0.2	0.5 ± 0.1	0.09 ± 0.01	0.60 ± 0.04
Loop 3	M219Q/F247N	Semi-reduced	0.5 ± 0.3	5.9 ± 0.5	n.a.	n.a.
Wild type		Oxidized	0.6 ± 0.5	7 ± 5	0.07 ± 0.02	7 ± 1
Wild type		Semi-reduced	0.3 ± 0.1	73 ± 5	n.a.	n.a.

Turnover values correspond to 4 μM electron donor.