Fig. 3.

Images of two-photon excitation of a mixed sample, in which each mammalian COS-7 cell only expresses one of either mAmetrine, TagRFPt or mKate2 fluorescent proteins. Cells were excited by the TL (left column), blue phase-shaped (middle column) and red phase-shaped (right column) pulses. Solid arrows, putative mAmetrine-expressing cells; open arrows, putative TagRFPt-expressing cells; solid arrowhead, putative mKate2-expressing cell. The TL pulses create higher image intensities than the shaped pulses do. To avoid saturation, lower PMT voltages have been applied to lower the fluorescence intensity of ~7 fold. Additionally, brightness and contrast of panels (a) and (b) have been scaled down ~10 fold to avoid saturation in display. Scale bar is 20 µm.