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A) Scheme for CHA as a real-time detector of linear RCA. B) Concentration dependence of Circular THTRCA of RCA-CHA at 60 °C in the ThermoPol reaction, using EHT-CHA and Bst large fragment polymerase. C) Concentration dependence of Circular TLTRCA of RCA-CHA at 37 °C in the Phi29 reaction, using EHT-CHA and Phi29 polymerase. The sequences and conditions used in these experiments are found in Tables S3 and S4 respectively.
