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. Author manuscript; available in PMC: 2014 May 22.
Published in final edited form as: J Am Chem Soc. 2013 May 9;135(20):7430–7433. doi: 10.1021/ja4023978

Figure 4.

Figure 4

High-temperature CHA transduction is sensitive and specific. A) 1% agarose gel of the product generated from a 2 hour RCA reaction with and without Circular THTRCA. B) The same RCA products from Figure A were diluted 4-fold with EHT-CHA, followed by a CHA reaction in ThermoPol reaction conditions at 60 °C. The concentrations listed are those before dilution. C) 1% agarose gel of the product generated from an overnight RCA reaction with and without Circular THTRCA. D) The same RCA products from Figure C were diluted 4-fold with HT-CHA, followed by a CHA reaction in ThermoPol reaction conditions at 60 °C. The concentrations listed are those before dilution.