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A) Scheme for CHA as a real-time detector of SDA. B) Concentration dependence of Circular THTSDA of SDA-CHA at 60 °C in the NEBuffer 2 reaction using EHT-CHA, Bst large fragment polymerase, and Nb. BsrDI. C) Concentration dependence of Circular TLTSDA of SDA-CHA at 37 °C in the NEBuffer 2 reaction using LT-CHA, Klenow (3’–5’exo-) polymerase, and Nb.BbvCI. The sequences and conditions used in these experiments are found in Tables S3 and S4 respectively.
